Aurum™ Total RNA Fatty and Fibrous Tissue Kit

RNA isolation / purification Tissue - Human Uterus

Experiment
RNA isolation / purification Tissue - Human Uterus
Product
Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories
Manufacturer
Bio-Rad Laboratories

Protocol tips

Upstream tips
- Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.
Protocol tips
- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step.

- If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit
Downstream tips
- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use.

- If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Publication protocol

Total RNA was isolated from myometrial biopsies by using the Aurum Total RNA Fatty and Fibrous Tissue Pack Kit (BioRad, Hercules, CA, USA) and reverse transcribed to generate cDNA by using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer's instructions. cDNA was stored at −20°C until PCR was performed. Forty-cycle PCR was performed on cDNA by using Taq PCR Master Mix (2X) (Affymetrix, Santa Clara, CA, USA) and primers (Table I) specific to NALCN, UNC80, UNC79, or NLF-1 (Integrated DNA Technologies, Coralville, IA, USA). Products were electrophoresed on a 1.5% agarose gel.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Uterus using Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories.

Paper title
Sodium leak channel, non-selective contributes to the leak current in human myometrial smooth muscle cells from pregnant women
Full paper
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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aurum™ Total RNA Fatty and Fibrous Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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